Confocal Microscopy

Confocal Microscope | Leica, TSC SP5 II

Location: LFG, Haberstraße 9a, 91058 Erlangen, Room U1.825

The Leica TCS SP5 confocal microscope covers a wide range of confocal and multiphoton imaging requirements with outstanding overall performance. The system offers the full range of scanning speeds at the highest resolution. With its proven, highly efficient SP detection (up to five channels simultaneously) and the optional AOBS (Acousto Optical Beam Splitter), the Leica TSC SP5 delivers bright, noise-free images with minimal photodamage at high speed.

Dr. Naveen Kumar

Microscopes: upright, inverted

Anti-vibration stage: with vibration damping

Continuous wave lasers: VIS, UV

Pulsed lasers: IR, VIS, UV

Optics:

  • Laser ports: up to 3 (UV – VIS – IR)
  • Number of lasers: up to 8
  • Excitation-emission splitting: Acousto-optical beam splitter (AOBS®), dichroic beam splitter
  • Detection range: 400 – 800 nm
  • UV and IR imaging: sequential or simultaneous
  • Extendable range: yes (UV, IR)
  • UV correction: individual precision correction optics (up to 5 positions)
  • Pinhole: single pinhole with stable alignment
  • Pinhole diameter control: motorized via software, automatic mode available
  • Scanning filter, optional: 458/514 nm, 488/561/633 nm

Scanner:

  • Scanning concept: optically correct scanning with low inertia
  • Choice between conventional and resonant scanner: conventional and resonant scanner in one system (optional)
  • Scanning speed granulation: 1400
  • Maximum frame rate 512 x 512: 5 Hz
  • Maximum frame rate 512 x 16: 50 Hz
  • Maximum image resolution: 8192 x 8192 pixels
  • Scan zoom: 1.0 …64 x

Internal confocal detection:

  • Emission separation: highly sensitive prism spectral detector
  • Maximum number of confocal channels: 5
  • Tunability of emission bands: yes
  • Spectral detection range: 400 to 800 nm
  • Tuning steps of emission bands: 1 nm
  • Minimal detection range: 5 nm
  • Sensor: high sensitivity low noise PMT: R 9624
  • Digitization: 12 or 18 bit per channel
  • Max. grey resolution: 16 bit imaging
  • Readout frequency: 40 MHz

External confocal detection:

  • Emission separation: user-exchangeable beam splitting filter cubes
  • Confocal channels: 2
  • Sensors: APDs made of PE (SPCM-AQRH series) or MPD (PDM series)
  • Quantum efficiency: PE APD: wavelength dependent, typ. 65% @ 670 nm, MPD APD: wavelength dependent, typ. 45% @ 550 nm
  • Dark counts: PE APD: < 250 cps, MPD APD: < 250 cps

Non-confocal detection:

  • Transmitted light detector: optional, allowing BF, DIC, Ph, etc.
  • Non descanned transmitted light channels: up to 4 channels (MP)
  • Non descanned reflect light channels: up to 4 channels (MP)

https://www.leica-microsystems.com/products/confocal-microscopes/p/leica-tcs-sp5/

Further information to follow